SOE-LRed: A simple and time-efficient method to localize genes with point mutations onto the Escherichia coli chromosome.
نویسندگان
چکیده
We use a powerful method to replace wild-type genes on the chromosome of Escherichia coli. Using a unique form of PCR, we generate easily constructible gene fusions bearing single point mutations. Used in conjunction with homologous recombination, this method eliminates cloning procedures previously used for this purpose.
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ورودعنوان ژورنال:
- Journal of microbiological methods
دوره 84 3 شماره
صفحات -
تاریخ انتشار 2011